FASCINATION ABOUT DOVITINIB

The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR goods have been digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A point mutation was released by the fast adjust web page-directed mutagenesis process ut